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Coding

Part:BBa_K1618011:Design

Designed by: Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-08-13)


Pro-GlgX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 2439
    Illegal EcoRI site found at 1557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 1557
    Illegal SpeI site found at 2440
    Illegal PstI site found at 2454
    Illegal NotI site found at 235
    Illegal NotI site found at 2447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 1557
    Illegal BamHI site found at 305
    Illegal BamHI site found at 1513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 2440
    Illegal EcoRI site found at 1557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 1557
    Illegal XbaI site found at 244
    Illegal SpeI site found at 2440
    Illegal PstI site found at 2454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 939


Design Notes

None


Source

Bacterial sequence.

References